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KMID : 0613820080180010058
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Journal of Life Science
2008 Volume.18 No. 1 p.58 ~ p.62
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Isolation and Characterization of a Marine Derived Bacterium Glaciecola sp. SL-12 Producing -agarase
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Lee Dong-Geun
Lee Ok-Hee
Jang Hyo-Jung
Jang Min-Kyung
Yoo Ki-Hwan
Lee Sang-Hyeon
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Abstract
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A novel agar-degrading bacterium SL-12 was isolated from seashore of Kijang at Busan, Korea, and cultured in marine broth 2216 media. Isolated bacterium SL-12 was identified as Glaciecola genus by 16S rDNA sequencing with 98% identity. The optimum pH of the enzyme activity was 7.0 and the optimum temperature for the reaction was . The enzyme hydrolyzed neoagarohexaose to yield neoagarobiose as the main product, indicating that the enzyme is -agarase. Thus, isolated bacterium and the enzyme would be useful for the industrial production of neoagarobiose.
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KEYWORD
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-agarase, marine, Glaciecola sp., neoagarobiose
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